Response Surface Methodology-Based optimization of Inulinase Production from New Bacillus Isolates

This study aimed to investigate new bacterial sources with the ability produce inulinase and optimize fermentation conditions for production. The production was carried out using newly isolated Bacillus licheniformis velezensis among identified sixteen strains. four essential variables incubation time (1-3 days), temperature (25-40°C), pH (5-7), Wheat Bran (WB) level (1-5%) determined by Plackett–Burman design (PB) were selected optimization studies Box-Benhken Design (BBD). These parameters found be very effective on maximum activities B. 401.18 EU/mL 344.61 EU/mL. Both inulinases sustained 50% of their initial activity at 30°C 9 days.. results point that bacteria are an important source as producer. isolates can used in industrial processes.